Isolation, identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight

In order to clarify the pathogenic bacteria species of Bletilla Striata leaf blight, the pathogen of B. Striata leaf blight was isolated, purified and identified by rDNA-ITS sequence analysis, the purified pathogen was grafted to B. Striata plants to evaluate its pathogenicity. The results showed that a total of 6 fungi strains were isolated from the diseased samples of B. striata, including three strains of Fusarium, namely: F. keratoplasticum, F. oxysporum and F. equiseti; a strain of Aspergillus japonicus; a strain of Botrytis cinerea; a strain of Mucor fragilis. According to the pathogenicity test, it was found that the above 6 strains of fungi were all pathogenic to B. Striata.